A A AText only | Accessibility | Privacy and cookies

Browse site A to Z

Search results

Go To Advanced Search

NIHR_RTB_PIS-ICF_v5.0_04APR2024_Localised

Description
PARTICIPANT INFORMATION SHEET The NIHR BioResource Research Tissue Bank Version 5.0 04th April 2024 *If you require
Url
/Media/Southampton-Clinical-Research/Downloads/NIHR-RTB-PIS-ICF-v5.0-04APR2024-Localised.pdf

NIHR_RTB_PIS-ICF_v5.0_04APR2024_Localised.cleaned

Description
PARTICIPANT INFORMATION SHEET The NIHR BioResource Research Tissue Bank Version 5.0 04th April 2024 *If you require
Url
/Media/Southampton-Clinical-Research/Downloads/NIHR-RTB-PIS-ICF-v5.0-04APR2024-Localised.cleaned.pdf

NIHR BioBank participant info sheet_v4

Description
PARTICIPANT INFORMATION SHEET The NIHR BioResource Research Tissue Bank Version 4.1 15th March 2023 *If you require
Url
/Media/Southampton-Clinical-Research/Downloads/NIHR-BioBank-participant-info-sheet-v4.pdf

NIHR_RTB_PIS-ICF_v4.1_15Mar2023Localised

Description
PARTICIPANT INFORMATION SHEET The NIHR BioResource Research Tissue Bank Version 4.1 15th March 2023 *If you require
Url
/Media/Southampton-Clinical-Research/Downloads/NIHR-RTB-PIS-ICF-v4.1-15Mar2023Localised.pdf

NIHR_RTB_PIS-ICF_v4.1_15Mar2023Localised

Description
2 PARTICIPANT INFORMATION SHEET The NIHR BioResource Research Tissue Bank Version 4.1 15th March 2023 *If you require this information to be translated into another language or in a different format, please speak to a member of the BioResource team We are inviting you to join the NIHR BioResource Research Tissue Bank, referred to as the ‘BioResource’. Many people live with health conditions such as diabetes, heart disease or mental health problems, which can have a major impact on their quality of life and on their family. We would like to invite you to join the BioResource, which supports research into health and medical conditions. Please read the following information carefully and discuss it with a friend or relative if you need to. Feel free to ask us if anything is not clear, or if you would like more information. You can also visit: https://bioresource.nihr.ac.uk/, which explains how to join the BioResource. What is the BioResource? The BioResource is a panel of hundreds of thousands of volunteers, with and without health conditions, who wish to participate in health research. The National Coordinating Centre is in Cambridge, with several BioResource centres around the country. Volunteers joining the BioResource are asked to donate a small blood or saliva sample and give consent to be invited to participate in health related research studies on the basis of data gathered from samples and information they have provided. Information and samples that the BioResource holds may also be made available to researchers working in biomedical and healthcare research. This extends the value of the BioResource enormously. More than half the studies we support just use samples and data we already hold. The BioResource is a unique and powerful resource for studying disease mechanisms and for investigating the links between our genes, the environment, health and disease. Through the BioResource researchers can study diseases or identify treatments. Why am I being asked to join? The BioResource is recruiting volunteers, both with and without health conditions. Do I have to join the BioResource? No. It is completely up to you to decide whether or not you wish to join. If you decide not to join, your decision will not affect the healthcare you receive in any way. If you join, you will be free to withdraw at any time, without having to give a reason. What will I need to do if I take part? * Read and sign a consent form; * Provide your name, date of birth, NHS/CHI number and contact details and allow us to store them; * Complete a questionnaire about your health and lifestyle; * Allow us to collect, store and analyse health information about you, including accessing your medical records, other health-related records, (for example, through NHS Digital or other databases), educational (if required) and social care records; * Donate a small sample of blood (3-4 teaspoons) or saliva; in the future, you may be invited to provide further samples; * Agree to be contacted in the future about research studies, including clinical trials, which we believe you are suitable for based on the information we hold. You will be provided with information about any study you are eligible for, and you can decide whether you would like to take part. You are under no obligation to do so. We will monitor how often you are approached. We are happy to contribute towards any travel costs. Some research will not need any further input from you as the samples and data you provide will be enough; * Agree to be contacted by the BioResource about updates, newsletters, invitations to open events, etc. You can choose not to receive these if you prefer. What are the disadvantages/risks? Qualified, experienced staff will collect blood samples. Blood sampling can sometimes cause minor discomfort, and there is a possibility that a small bruise may develop. What will happen to the samples I give? We want to know how genes influence health and disease. Genes are made up of DNA (deoxyribonucleic acid). We will extract DNA from your blood or saliva sample and study your genetic makeup. We may also test other components of your blood, such as cells, RNA, protein and metabolites. Your samples will be kept in a secure location. Will my details be kept confidential? Yes. Best ethical and legal practices will be followed to ensure that all information collected about you will be handled safely and securely. Your personal details will never be released to researchers without your consent. Your samples will be labelled with a unique number before being transferred to the laboratory for testing. Data about you (e.g. personal details and health information) will be stored in secure electronic databases. Any information from genetic and other tests or analyses will be stored separately from your personal details. Access to your personal details will only be available to authorised members of the BioResource (for example, to invite you to take part in other research studies). Your samples and data can only be accessed by researchers once they’ve been de-personalised (i.e. with all identifying information removed). Information from these tests will not be used or made available for any purpose other than for research. Researchers can apply to access your samples or data, and they have to explain how they will use it, e.g. the research question they wish to answer. This is referred to as ‘managed access’. Data may be accessed in a number of ways, for example, by logging in to secure data analysis environments or by downloading from the BioResource. Researchers will be reminded of their obligation to keep your data safe and secure by accepting the terms of a data transfer/access agreement. Researchers who analyse the information should not be able to identify you, and promise not to try to do so as a condition of data access. ‘Managed access’ requests could come from researchers from the public and charitable sector (Universities, Research Institutes) or from commercial and pharmaceutical companies. These can be based either in the UK or overseas. The BioResource will request further details about you from public bodies, like the NHS (via NHS Digital or other registries) and the UK Health Security Agency. To do this, identifiable information, like your name, NHS number and date and birth, will be sent securely to these agencies, so that they can find you in their databases. Researchers can share the results of their studies in reports or publications which are published on the internet and in press articles, etc. Under no circumstances will information that identifies you personally be disclosed in any of these documents. An explanation of how the BioResource complies with the General Data Protection Regulation (GDPR) can be found at the end of this information leaflet. The National Genomic Research Library The National Genomic Research Library is a secure national database of de-identified genomic and health data that is managed by Genomics England, which is a company set-up by the Department of Health and Social Care. Approved researchers can use the samples and data in a form that does not identify you to study diseases and look for new treatments. Adding your personal health and genomic data to the Library helps by adding to others’ data to make the Library a rich source of data for researchers. Everyone is unique and the more the Library holds, the better the outcomes for everyone. If you agree to take part in the Library, we will provide basic identifiers such as your name, date of birth, postcode and NHS Number to NHS Digital and other organisations so that they may collate your medical history records and provide the data to the Library. All identifying information is removed before researchers are able to use it. Your data will be kept in secure systems. Your data cannot be removed from the Library and any results of research that are taken out cannot be used to re-identify you. Your data may include: * Electronic copies of all your past and future records from the NHS, your GP and other organisations (such as NHS Digital and Public Health bodies); * Information about any illnesses or stays in hospital; * Copies of hospital or clinic records, medical notes, social care, and local or national disease registries, and data from other research studies; * Relevant images from your NHS records, such as MRI scans, X-rays, or photographs; * Data from other research registries and studies that may be relevant (but only where you have given them your permission to share that information). Only researchers who are trying to better understand diseases and how to treat them will have access to the National Genomic Research Library. Researchers may come from all over the world, pooling international data and research gives the best chance of new discoveries. Approved researchers may work for not-for-profit organisations, such as research charities, universities, or hospitals, and for-profit (commercial) companies such as drug or technology companies. They will only have access to your de-identified genomic and health data in the National Genomic Research Library if they apply and are approved by Genomics England. You can leave the Library at any time without giving a reason. You will need to sign a withdrawal form to record your decision. The form can be requested from Genomics England. Can I know the results obtained from my study samples? The BioResource does not provide a report or feedback on any genetic or laboratory results obtained from your sample, as it is taken for research purposes only. Will any of the results obtained with my sample predict the risk of disease? We cannot answer this question. Many of the studies that the BioResource supports aim to discover variations in the genetic code (‘genetic markers’) that might predict the risk of certain diseases. However, it may be years before researchers can confirm how important or accurate these markers are and determine the associated risk. If you are contacted to take part in a future study, this does not mean that your health is at risk. Genetic variation between individuals can both protect and put individuals at risk of disease. We are a long way from understanding how they balance each other and what combination of markers combined with other factors such as age, gender, smoking and drinking habits, increases the risk of disease. Some of the studies the BioResource may invite you to take part in could reveal unexpected/previously unknown health conditions or future health risks. You will have the opportunity to confirm if you are happy to be invited to these studies at the time. What happens if an invention is made using my sample? Your donated samples and related information are given as a gift, which may help researchers in the public and commercial sector make an invention that could improve the future healthcare of patients and blood/organ donors, such as a new product to diagnose or treat a condition. If an invention results from the research undertaken with your sample, you will not receive any compensation, recognition or payment. The BioResource will not share your sample to make a profit. Published studies are available to view on the NIHR BioResource website https://bioresource.nihr.ac.uk/studies/ Withdrawal process If you choose to withdraw, you have two options. 1. You can allow us to continue accessing your healthcare records (and any required social and educational records) from central NHS records, your hospital, your GP, school and/or research done with your sample. This is a ‘no further contact’ withdrawal. 2. You can ask us to stop further use of your data and to destroy your remaining samples. Any research that has used your data or sample(s) cannot be undone. This is ‘no further use’ withdrawal. Your personal information will be retained in an archive so that a record remains of your initial consent and the withdrawal process. If the BioResource is unable to confirm your decision, your sample(s) and data will be retained for future use, and you will not be contacted again. Who funds and manages the BioResource? The BioResource is funded by the National Institute for Health and Care Research (NIHR). The Research Tissue Bank has been reviewed and given a favourable opinion by the East of England - Cambridge Central Research Ethics Committee. The BioResource is led by Professor John Bradley (Chief Investigator) and Nathalie Kingston PhD (Director). The organisation responsible for the management of the NIHR BioResource Research Tissue Bank is Cambridge University Hospitals NHS Foundation Trust. Further information If you require further information before deciding to join the BioResource, or have any queries, please feel free to contact the BioResource team on 0800 090 2233 or email us on nbr@bioresource.nihr.ac.uk; or contact your local NIHR BioResource Southampton team on 023 8120 4989 or email UHS.recruitmentCRF@nhs.net Thank you for reading this information sheet and for considering joining the BioResource. 2 of 10 Website: https://bioresource.nihr.ac.uk/ Email: nbr@bioresource.nihr.ac.uk IRAS ID: 313104 NIHR_RTB_PIS-ICF_v4.1_15MAR2023 General Data Protection Regulation (GDPR) Here, we explain how we comply with the General Data Protection Regulation (GDPR) (Regulation (EU) 2016/679). Please also read our https://bioresource.nihr.ac.uk/media/04nhduml/privacy-notice-v2.pdf, which explains what we do with the information we hold about you, how you can request access to this personal data, and other data rights. Cambridge University Hospitals NHS Foundation Trust (CUH) is responsible for managing the NIHR BioResource Research Tissue Bank. We will be using information from you, and your healthcare, social care and education (if required) records in order to support research studies, and CUH will act as the data controller for the information we hold. We are responsible for looking after your information and using it properly. Cambridge University Hospitals NHS Foundation Trust will keep identifiable information about you for 10 years after the study has finished, and we may approach you to extend this. How will we use information about you? We, the NIHR BioResource, will use information from you, from your medical records, including from your GP, hospital records and other health-related central records for this research project. The NIHR BioResource will also access your social care and if required, your education records. This information will include your: * Full name; * NHS/CHI number; * Date of birth; * Contact details, including address, phone number and email address; * Name and contact details of your GP; * Health-related information, e.g., on your lifestyle, disease history, medication etc; * Social care and education information, e.g., level of education; * Genetic information that will be generated from your blood or saliva samples or provided by, for example, NHS health-related central records, disease registries etc. People will use this information to do the research or to check your records to make sure that the research is being done properly. People who do not need to know who you are will not be able to see your name, NHS/CHI number, date of birth or contact details (which are your “personal identifiable information”). Your data will have a code number instead. We will keep all information about you safe and secure. Information about you, but not your personal identifiable information, will be sent to researchers in other countries worldwide. They must follow our rules about keeping your information safe. If and when the NIHR BioResource finishes, we will keep some of the data so we can check the results. We will write our reports in a way that ensures no-one can work out that you took part in the study. What are your choices about how your information is used? * You can stop being part of the study at any time, without giving a reason; * If you choose to stop taking part in the study, you have two options as outlined in the ‘Withdrawal process’ section. Further information on leaving the BioResource can be found here: https://bioresource.nihr.ac.uk/participants/members/leave-us/; * You may request a copy of the information we hold about you; * We need to manage your records in specific ways for the research to be reliable. We will be able to change some of the information we hold about you (for example, your contact details and contact preferences) if you ask us to; however, we won’t be able to let you change other data we hold about you (such as your genetic information). Where can you find out more about how your information is used? You can find out more about how we use your information * At www.hra.nhs.uk/information-about-patients/ * Our privacy notice available from https://bioresource.nihr.ac.uk/media/04nhduml/privacy-notice-v2.pdf which includes the contact details of the Data Protection Officer at CUH; * By asking one of the research team; * By sending an email to nbr@bioresource.nihr.ac.uk; * By calling us on Freephone 0800 090 2233. 8 of 11 Website: https://bioresource.nihr.ac.uk/ Email: nbr@bioresource.nihr.ac.uk IRAS ID: 313104 NIHR_RTB_PIS-ICF_v4.1_15MAR2023 Attach barcode here PARTICIPANT CONSENT FORM Please initial box The NIHR BioResource Research Tissue Bank Version 4.1 15th March 2023 1. I confirm that I have read (or had read to me) the information sheet 15/MAR/2023 version 4.1. I have had the opportunity to consider the information, ask questions and have these answered satisfactorily. 2. I understand that my participation is voluntary and that I am free to withdraw at any time, without giving any reason, and without my medical care or legal rights being affected. 3. I agree that the BioResource may access my medical, health, social care, and educational (if required) records held by public bodies, and to analyse and store this information long-term. I understand that this may include the provision of information about my health status. I understand that in order to access these records, identifiable personal details – like name, NHS number, date of birth will be sent to these public bodies. 4. I agree to give blood or saliva for health related research. I understand that my samples may be shared to support future research, including commercial studies. I understand my samples will be tested, and that this may include the reading of my entire genetic code. I understand my sample will be stored long-term. 5. I agree to provide personal and contact details. I agree to provide information for example, through the completion of questionnaires. I agree to be contacted by the BioResource to invite me to participate in other research studies. 6. I understand that relevant sections of my medical/health related, social care and education (if required) records and data collected during the study may be looked at by approved individuals for auditing and monitoring purposes. I give permission for this. 7. I understand my samples and de-personalised data may be shared with researchers through a ‘managed access’ process. 8. I understand that my samples are taken for research, and the BioResource will not feed back any genetic or other test results. 9. I agree to join the NIHR BioResource Research Tissue Bank. CONSENT TO THE NATIONAL GENOMIC RESEARCH LIBRARY OPTIONAL: Please put your initials in ONE box only This section gives you the choice to join the National Genomic Research Library 10. I understand that my data will be held by the National Genomic Research Library and I give my permission for researchers to access my data for approved research. I understand I can withdraw from the National Genomic Research Library at any time. I agree for my data to be held by the National Genomic Research Library. Please initial the box. OR I DO NOT want my data to be held by the National Genomic Research Library. Please initial box. ……………………………………………. ……………… ……………………… …………….. Participant: First Name and Surname Date of Birth Signature Date (BLOCK CAPITALS) ……………………………………………………………… ……………………… ……………... Person receiving consent: First Name and Surname Signature Date (BLOCK CAPITALS) FOR WITNESS AND/OR TRANSLATOR USE ONLY WITNESS/TRANSLATOR STATEMENT: If participant is not able to read the text and/or sign for themselves but has capacity to give consent OR if the Participant Information Sheet and Consent Form has been translated. Witness/translator [to delete either as appropriate]: I witnessed accurate reading of the consent form to the potential participant, who could ask any questions and received satisfactory answers or I was present during the meeting between [insert name] and the participant. I translated for the participant the Participant Information Sheet and the Consent Form. I confirm that they gave their consent freely. ……………………………………………………. ……………………… …………….. Witness/translator: First Name and Surname Signature Date (BLOCK CAPITALS) 11 of 11 Website: https://bioresource.nihr.ac.uk/ Email: nbr@bioresource.nihr.ac.uk IRAS ID: 313104 NIHR_RTB_ICF-PIS_v4.1_15MAR2023 When completed: 1 for participant; 1 (original) for researcher site file; [1 to be kept in medical notes (optional – delete as required)].
Url
/Media/Southampton-Clinical-Research/Downloads/NIHR-RTB-PIS-ICF-v4.1-15Mar2023Localised.docx

VAC31518COV2001_UK_Parent and Guardian ICF_V2.0_15Feb2021_Clean Localised (1)

Description
Southampton NIHR Clinical Research Facility University Hospital Southampton NHS Foundation Trust C Level Wes
Url
/Media/Southampton-Clinical-Research/Downloads/VAC31518COV2001-UK-Parent-and-Guardian-ICF-V2.0-15Feb2021-Clean-Localised-1.pdf

Laboratory medicine investigations rev 25

Description
University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a UHSFT Pathology Laboratory Medicine List of Investigations For information on the basis of reference intervals or decision limits, please contact the laboratory Please note multiple requests and cascade requests have a cumulative effect on turnaround times. Please contact the laboratory if an expected result time is required. DEPARTMENT/TEST Section / Area code Units TAT days(working) Special requirements CHEMICAL PATHOLOGY RAPID TURNAROUND – SEND ONLY 6 ML SERUM SEPARATOR GEL (SST BUFF TOP) BLOOD SAMPLE FOR ALL TESTS UNLESS OTHERWISE STATED. FOR PAEDIATRIC/CHILDREN SEND FULL 2 ML PAEDIATRIC LITHIUM HEPARIN BLOOD SAMPLE RENAL PROFILE SODIUM Automated mmol/L <1 POTASSIUM Automated mmol/L <1 CREATININE Automated umol/L <1 BONE PROFILE CALCIUM (actual NOT adjusted) ALBUMIN Automated mmol/L <1 Automated g/L <1 TOTAL PROTEIN Automated g/L <1 LIVER PROFILE ALT Automated iu/L <1 ALBUMIN Automated g/L <1 ALP Automated iu/L <1 BILIRUBIN (TOTAL) Automated umol/L <1 TOTAL PROTEIN Automated g/L <1 Accreditation Status Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Revision 25 Page 1 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST Section / Area code OTHER AUTOMATED LABORATORY TESTS (EXCLUDING URINE TESTS) ACE Automated AFP Automated ALPHA-1 ANTITRYPSIN Automated AMMONIA Automated AMYLASE Automated AST Automated BICARBONATE Automated BILE ACIDS Automated BILIRUBIN - DIRECT Automated BILIRUBIN - NEONATAL Automated CA 125 Automated CA19-9 Automated CARBAMAZEPINE Automated CEA Automated CHLORIDE Automated CHOLESTEROL Automated CK (creatine kinase) CORTISOL (PLASMA) C-REACTIVE PROTEIN (CRP) DIGOXIN Automated Automated Automated Automated ETHANOL (alcohol) FERRITIN Automated Automated Units iu/L ku/L g/L umol/L iu/L iu/L mmol/L umol/L umol/L umol/L ku/L ku/L mgl/L ug/L mmol/L mmol/L iu/L nmol/L mg/L nmol/L mg/100ml ug/L Revision 25 TAT days(working) Special requirements Accreditation Status <1 SST or CLOTTED Accredited <1 Accredited 2 SST Accredited 1 EDTA-SEND IMMEDIATELY Accredited <1 Accredited <1 Accredited <1 Accredited <1 SST Accredited <1 Accredited <1 Accredited 1 SST Accredited 1 SST Accredited <1 Collect trough level Accredited 1 SST Accredited <1 Accredited Accredited <1 <1 1 SST <1 Accredited Accredited Accredited <1 COLLECT AT LEAST 6 HOURS Accredited POST DOSE 7 FLUORIDE/OXALATE Accredited <1 Accredited Page 2 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST FOLATE (PLASMA) FREE T3 FREE T4 FSH GAMMA GT GLUCOSE HAPTOGLOBIN hCG HDL CHOLESTEROL IRON LDH LH LITHIUM MAGNESIUM METHOTREXATE OESTRADIOL OSMOLALITYSERUM/PLASMA PARACETAMOL PHENOBARBITONE PHENYTOIN PHOSPHATE PROGESTERONE PROLACTIN PSA Revision 25 Section / Area code Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Automated Units ug/L pmol/L pmol/L iu/L iu/L mmol/L g/L iu/L mmol/L umol/L iu/L iu/L TAT days(working) Special requirements <1 <1 <1 <1 <1 <1 FLUORIDE OXALATE <1 SST <1 <1 <1 <1 <1 Accreditation Status Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited mmol/L <1 mmol/L umol/L pmol/L <1 4 hours <1 mOsm/KgH2O <1 mg/L <1 mg/L <1 mg/L <1 mmol/L <1 mmol/L <1 mu/L <1 ug/L <1 SST or CLOTTED. Collect at least Accredited 10 hrs post dose Accredited SST Accredited Accredited Accredited collect trough level collect trough level Accredited Accredited Accredited Accredited Accredited Accredited Accredited Page 3 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST PTH SALICYLATE Section / Area code Automated Automated Units pmol/L mg/L TAT days(working) Special requirements Accreditation Status 2 LI/HEP - SEND TO LAB WITHIN 8 Accredited HOURS <1 Accredited SHBG Automated nmol/L 1 SST Accredited TESTOSTERONE Automated nmol/L <1 SST Accredited THEOPHYLLINE Automated mg/L <1 collect trough level Accredited TRANSFERIN Automated g/L <1 Accredited TRIGLYCERIDES Automated mmol/L <1 Accredited TROPONIN - I TSH Automated ng/L <1 Automated mu/L <1 SST (routine requests) OR BD Rapid Serum tube (Emergency Department Low risk chest pain pathway ONLY) Accredited Accredited URATE (uric acid) Automated umol/L <1 Accredited UREA (nitrogen) Automated mmol/l <1 Accredited VALPROATE Automated mg/L <1 collect trough level Accredited VITAMIN B 12 Automated ng/L <1 Accredited VITAMIN D, 25-OH VITAMIN Automated D (25-OH- (routine method) nmol/L <1 CHOLECALCIFEROL) GENERAL BIOCHEMISTRY TESTS (including urine tests) 17 OH PROGESTERONE - LC-MS BLOOD SPOT nmol/L 11 17 OH PROGESTERONE – LC-MS SERUM nmol/L 5 5 –HIAA Chromatography umol/24 hours5 ACTH Special Biochem ng/l 2 Accredited SST GUTHRIE CARD Not Accredited SST OR HEPARIN Accredited 24h URINE ONLY – PRESERVED Accredited WITH GLACIAL ACETIC ACID EDTA PLASMA TO BE Accredited RECEIVED IN LAB TO BE Revision 25 Page 4 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST Section / Area code Units ACYL CARNITINES (BLOOD LC-MS SPOT) ALBUMIN (URINE) Automated N/A mg/L ALDOSTERONE Special Biochem pmol/L ALK PHOS ISO ENZYMES ALPHA-1 AT PHENOTYPE AMINO ACIDS (CSF) AMINO ACIDS (PLASMA) AMINO ACIDS (URINE) – FOR CYSTINURIA ONLY ANDROSTENEDIONE (SERUM) C-PEPTIDE CAERULOPLASMIN CALCITONIN CALCIUM (URINE) CARBOXYHAEMOGLOBIN Special Biochem qualitative Special Biochem qualitative Chromatography umol/L Chromatography umol/L Chromatography umol/mmol creat Chromatography nmol/L Special Biochem pmol/L Automated mg/L Special Biochem ng/L Automated mmol/spec mmol/spec mmol/mmol crea Critical Care Lab % TAT days(working) Special requirements Accreditation Status SEPARATED AND FROZEN WITHIN 24 HOURS 5 GUTHRIE CARD (from Li Hep plasma Accredited 1 RANDOM URINE Accredited EDTA IF RENIN ALSO REQUIREDAccredited 5 SST OK IF ALDO REQUEST ONLY 10 SST Accredited 10 SST Accredited 5 CSF Accredited 5 LI/HEP or SST Accredited 5 RANDOM URINE Accredited 5 SST Accredited 5 SST OR LI/HEP - SEND TO LAB Accredited WITHIN 2 HOURS 2 SST Accredited 2 FASTING A.M. PLAIN SAMPLE / Accredited SST / Li HEP Accredited 1 24h PREFERRED, CHILDREN RANDOM Whole blood Lithium Heparin – Accredited 1 Send to Main Lab. CHOLINESTERASE PHENOTYPE CITRATE (URINE) Send Away Automated 10 mmol/spec 6 SST or CLOTTED 24h URINE COLLECTION Accredited Accredited Revision 25 Page 5 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST Section / Area code Units TAT days(working) Special requirements Accreditation Status CITRATE (RANDOM URINE) Automated mmol/mmol creat 6 RANDOM URINE Accredited CORTISOL (BLOOD SPOT) Special Biochem nmol/L 10 GUTHRIE CARD Not Accredited CORTISOL (SALIVA) Special Biochem nmol/L 10 SALIVA Accredited CORTISOL (URINE) Special Biochem nmol/spec 5 24h URINE COLLECTION Accredited CREATININE CLEARANCE Automated mL/min 1 24h URINE COLLECTION AND Accredited SST CREATININE (URINE) Automated mmol/L 1 URINE Accredited CREATININE (FLUID) Automated umol/L 1 24h URINE COLLECTION Accredited CICLOSPORIN Automated ug/L 1 EDTA Accredited CYSTINE Chromatography umol/mmol creat 10 URINE Accredited DHEA-SULPHATE Chromatography umol/L 5 SST Accredited DRUG SCREEN Toxicology 7 RANDOM URINE Accredited ELF test (enhanced liver fibrosis) Special Biochem None SST / SERUM ONLY REQUIRES HA, P3NP & TIMP ANALYSIS Accredited ERYTHROPOIETIN (EPO) Special Biochem IU/L 2 SST Accredited FAECAL ELASTASE Special Biochem ug E1/g stool 5 RANDOM FAECES Accredited FAECAL IMMUNOCHEMICAL TESTING (FIT) Special Biochem ug Hb /g Stool 11 FIT sample Collection KIT Accredited GROWTH HORMONE (SERUM) Special Biochem ug /L 2 SST Accredited Special Biochem Lithium heparin plasma or EDTA Accredited plasma. Patients must be fasting Homocysteine umol/l 5 and the sample separated and frozen at -20 C within 30 minutes of collection. Revision 25 Page 6 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST HAEM PIGMENTS Section / Area code Automated Units Abs Unit TAT days(working) Special requirements 4 hours Accreditation Status Accredited HMMA & HVA (neuroblastoma screen in children) Chromatography umol/mmol creat 5 Special Biochem HYALURONIC ACID ug/L 5 Accredited RANDOM URINE - Frozen if sent from an external laboratory. SST ONLY Accredited HYDROXYBUTYRATE IGF-1 INSULIN LACTATE (BLOOD) Toxicology mmol/L 12 Special Biochem nmol/L 2 Special Biochem mu/L 5 Critical Care Lab mmol/L 1 LACTATE (CSF) Critical Care Lab mmol/L 1 MAGNESIUM (URINE) Automated mmol/L 1 METANEPHRINES - URINE Chromatography umol/24 hr (Normetanephrine, Metanephrine and 3- or umol/mmol 5 Methoxytyramine) creatinine METHAEMALBUMIN Automated 2 Critical Care lab METHAEMOGLOBIN % 2 CLOTTED Accredited SST Accredited SST - SEND TO LAB WITHIN 2 Accredited HOURS Lithium heparin or SST – SEND TO Accredited LAB WITHIN 10 MINUTES of venesection. Plain sample only (no Accredited anticoagulant) - SEND TO LAB WITHIN 10 MINUTES of venesection 24 HR URINE COLLECTION: Bottle preserved with 20mL 6M HCl. 24h URINE COLLECTION IN ACETIC ACID (Adults) or RANDOM URINE (Children, for phaeochromocytoma screen only) LI/HEP Accredited Whole blood Lithium Heparin – Accredited Send to Main Lab Accredited Accredited Revision 25 Page 7 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST MICROSCOPY (FAECAL, FAT GLOBULES, PAEDIATRIC) NT-ProBNP ORGANIC ACIDS (URINE) Section / Area code Automated Units Special Biochem ng/L Chromatography OSMOLALITY - URINE OXALATE (TIMED URINE) Automated Automated mosmol/kg (H20) mmol/24h Automated OXALATE (RANDOM URINE) mmol/mmol creat P3NP (Intact N-terminal propeptide of type III collagen) Special Biochem ug/L Chromatography pH (FAECES) pH units pH (URINE) Chromatography pH units PHENYLALANINE (BLOODSPOT) Send Away µmol/L Chromatography PLASMA METANEPHRINES pmol/L PORPHOBILINOGEN PORPHYRIN (URINE) PROTEIN CREATININE RATIO (URINE) Automated Send Away Automated qualitative nmol/L g/L TAT days(working) Special requirements 3 FRESH FAECES Accreditation Status Accredited 2 MUST BE Lithium Heparin Plasma Accredited 5 RANDOM URINE, FRESH Accredited 1 RANDOM URINE Accredited 6 24h URINE COLLECTION Accredited 6 RANDOM URINE Accredited 5 SST ONLY Accredited A MINIMUM 1g OF FAECES.W Accredited HICH MUST REACH THE 5 LABORATORY AND BE HANDED DIRECTLY TO A MEMBER OF STAFF WITHIN 1 HOUR OF COLLECTION. 1 FRESH SAMPLE REQUIRED Accredited 2 MONITORING KNOWN CASES Accredited OF PHENYLKETONURIA ONLY Send immediately. Samples to be Accredited 5 separated and frozen within 30 minutes of collection. 1 RANDOM URINE, FRESH PROTECT FROM LIGHT Accredited 1 EARLY MORNING URINE Accredited Accredited Revision 25 Page 8 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST PROTEIN (URINE) PROTEIN (CSF) RENIN SOMATOMEDIN C (IGF-1) STONE ANALYSIS SWEAT CHLORIDE Section / Area code Units Automated mg/L Automated mg/L Special Biochem mU/L Special Biochem nmol/L Special Biochem Trace Elements mmol/L SOLUBLE TRANSFERIN RECEPTOR TACROLIMUS TIMP-1 (Tissue inhibitor of matrix metalloproteinase-1) THYROGLOBULIN THYROGLOBULIN ANTIBODIES TYROSINE (BLOODSPOT) nmol/L Send Away ug/L Special Biochem µg/L Special Biochem ug/L Special Biochem KU/L Chromatography µmol/L VITAMIN A Chromatography μmol/L Chromatography VITAMIN E μmol/L POINT OF CARE TESTS Revision 25 TAT days(working) Special requirements 1 RANDOM URINE 1 CSF IN PLAIN CONTAINER 5 EDTA, SEP, FREEZE WITHIN 30 MINS 1 SST Accreditation Status Accredited Accredited Accredited Accredited 10 RENAL CALCULUS Accredited Accredited 2 WESCOR MACRODUCT 30 EDTA Accredited 4 Accredited SST ONLY Accredited 5 ONLY available as part of ELF test 2 SST Accredited 2 SST Accredited MONITORING KNOWN CASES Accredited 3 OF PHENYLKETONURIA OR TYROSINAEMIA ONLY 5 SST Accredited SST - Vitamin E concentration is Accredited affected by plasma lipids. Vitamin E 5 to total cholesterol ratio is a better indicator of status (Adult ref: 1.3- 16.2 umol/mmol) Page 9 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST Section / Area code Units TAT days(working) Special requirements Accreditation Status BLOOD GAS (Arterial, VenousPOCT or Capillary whole blood) N/A 1 Heparinised syringe on blood gas analyser. Whole blood Lithium Heparin – CARBOXYHAEMOGLOBIN POCT % Saturation 1 SEND TO LAB or Heparinised syringe on blood-gas analyser. Lithium heparin or SST – SEND TO LACTATE (BLOOD) mmol/L 1 LAB WITHIN 10 MINUTES of venesection or Heparinised syringe POCT on blood-gas analyser Plain sample only (no LACTATE (CSF) POCT mmol/L 1 anticoagulant) - SEND TO LAB WITHIN 10 MINUTES of venesection METHAEMOGLOBIN POCT % 2 Whole blood Lithium Heparin – SEND TO LAB or Heparinised syringe on blood-gas analyser. TRACE ELEMENT LABORATORY TESTS Trace Elements 6ml Greiner Trace Element Na Hep Accredited ALUMINIUM umol/L 5 tube (Royal blue top) ANTIMONY (BLOOD) ANTIMONY (URINE) ARSENIC (BLOOD) Trace Elements 5 Trace Elements 5 Trace Elements umol/L 5 Adults: 6ml Greiner Trace Element Accredited Na Hep tube (Royal blue top) Paed: 2ml Teklab Hep TE tube Accredited 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Lithium heparin, sodium heparin or Accredited EDTA Revision 25 Page 10 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST ARSENIC (URINE) BARIUM BERYLLIUM (URINE) BISMUTH (BLOOD) BISMUTH (URINE) BORON (PLASMA) BORON (URINE) CADMIUM (BLOOD) CADMIUM (URINE) CHROMIUM (BLOOD) CHROMIUM (PLASMA) CHROMIUM (URINE) COBALT (BLOOD) COBALT (PLASMA) COBALT (URINE) Revision 25 Section / Area code Units Trace Elements Trace Elements nmol/L Trace Elements nmol/L Trace Elements nmol/L Trace Elements Trace Elements umol/L Trace Elements Trace Elements nmol/L Trace Elements Trace Elements nmo/L Trace Elements nmo/L Trace Elements Trace Elements nmol/L Trace Elements nmol/L Trace Elements TAT days(working) Special requirements Accreditation Status 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 Lithium heparin or EDTA Accredited 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Page 11 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST COPPER (PLASMA) COPPER (URINE) GOLD (PLASMA) GOLD (URINE) IODINE (URINE) IRON (URINE) LEAD (BLOOD) LEAD (URINE) MANGANESE (BLOOD) MERCURY (BLOOD) MERCURY (URINE) MOLYBDENUM (PLASMA) Section / Area code Units Trace Elements umol/L Trace Elements umol/24hr Trace Elements umol/L Trace Elements Trace Elements umol/L Trace Elements umol/24 hr Trace Elements umol/L Trace Elements nmol/mmol creatinine Trace Elements nmol/L Trace Elements nmol/L Trace Elements nmol/mmol creatinine Trace Elements nmo/L TAT days(working) Special requirements Accreditation Status Adults: 6ml Greiner Trace Element Accredited Na Hep tube (Royal blue top) 4 Accredited Paed: 2ml Teklab Hep TE tube 24 hour collection in a 2.5 litre Accredited 5 container with no preservative added. 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 24 hour collection in a 2.5 litre Accredited 5 container with no preservative added. 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Adults: 6ml Greiner Trace Element Accredited Na Hep tube (Royal blue top) 5 Paed: 2ml Teklab Hep TE tube Accredited 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 2ml Teklab Li Hep Trace Element Accredited tube Revision 25 Page 12 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST MOLYBDENUM (BLOOD) Section / Area code Units Trace Elements nmo/L NICKEL (PLASMA) Trace Elements nmol/L NICKEL (URINE) Trace Elements PLATINUM (BLOOD) Trace Elements nmol/L PLATINUM (URINE) Trace Elements Trace Elements RELATIVE EXCHANGABLE COPPER (PLASMA) % SELENIUM (PLASMA) Trace Elements umol/L SELENIUM (URINE) SILVER (BLOOD) SILVER (URINE) STRONTIUM (PLASMA) THALLIUM (BLOOD) THALLIUM (URINE) Revision 25 Trace Elements Trace Elements nmol/L Trace Elements Trace Elements umol/L Trace Elements nmol/L Trace Elements TAT days(working) Special requirements Accreditation Status 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Adults: 6ml Greiner Trace Element Accredited 4 Na Hep tube (Royal blue top) Paed: 2ml Teklab Hep TE tube Send immediately Adults:6ml Greiner Trace Element Accredited Na Hep tube (Royal blue top) 5 Paed: 2ml Teklab Hep TE tube Accredited 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 Lithium heparin, sodium heparin or Accredited EDTA 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Page 13 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST TIN (PLASMA) TIN (URINE) TITANIUM (PLASMA) TITANIUM (URINE) VANADIUM (URINE) URANIUM (URINE) ZINC (PLASMA) Section / Area code Units Trace Elements umol/L Trace Elements Trace Elements nmol/L Trace Elements Trace Elements nmol/L Trace Elements pmol/L Trace Elements µmol/l Trace Elements TOXIC METALS SCREEN (BLOOD) Trace Elements TOXIC METALS SCREEN (URINE) Revision 25 TAT days(working) Special requirements Accreditation Status 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection 5 20ml ‘Sterilin’ Universal container, Accredited or 24hr collection Adults: 6ml Greiner Trace Element Accredited 4 Na Hep tube (Royal blue top) Accredited Paed: 2ml Teklab Hep TE tube 6ml Greiner Trace Element Na Hep Accredited tube (Royal blue top) Please send with paired random 2 urine sample. Blood sample is primary sample required for TMS analysis. Urine will only be tested if required. 20ml ‘Sterilin’ Universal container Accredited Please send with paired whole blood sample. Blood sample is 2 primary sample required for TMS analysis. Urine will not be tested unless received with a blood sample. Page 14 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST HAEMATOLOGY ESR FULL BLOOD COUNT – INCLUDING: HAEMOGLOBIN WBC - WHITE CELL COUNT RBC - RED CELL COUNT PLATELETS PCV MCV MCH MCHC NEUTROPHILS LYMPHOCYTES EOSINOPHILS BASOPHILS MONOCYTES BLOOD FILM G6PD SCREEN PYRUVATE KINASE Units TAT Special Lab sample days(working) requirements storage time mm/1st hour <1 <1 g/L x10^9/L x10^12/L x10^9/L L/L fl pg g/L x10^9/L x10^9/L x10^9/L x10^9/L x10^9/L Normal/deficient 2 Normal/deficient 30 2mL EDTA. 36-48 hours Please ensure maximum fill EDTA 36-48 hours 1 week EDTA 3 days Sent away to KingsPath Accreditation Status Accredited Accredited Accredited Accredited Accredited Revision 25 Page 15 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE IM SCREEN Pos/Neg 1 EDTA 36-48 hours MALARIAL SCREEN Pos/Neg <1 EDTA 36-48 hours ABSOLUTE RETICULOCYTES x10^9/L <1 EDTA 36-48 hours SICKLE SCREEN Pos/Neg <1 EDTA 3 days CSF CYTOSPIN HBA1c Cells present/absent <1 mmol/mol 2 CSF EDTA 1 week 36-48 hours HAEMOSTASIS (1 x 2.7 ml citrate (blue top) bottle required unless otherwise indicated) HEPARIN (ANTI-Xa ASSAY) for monitoring LMW Heparin Anti Xa assay for Rivaroxaban Anti Xa assay for Apixaban See report 2 Anti Xa assay for Edoxaban 1 day Deliver straight to lab Accredited Accredited Accredited Accredited Accredited Accredited Accredited Dilute Thrombin Time assay for Dabigatran D-DIMER ASSAY ugFEU/ml <1 FACTOR X ASSAY u/dL 30 FACTOR XI ASSAY u/dL 30 FACTOR XII ASSAY u/dL 30 FACTOR II ASSAY u/dL 30 FACTOR V ASSAY u/dL 30 FACTOR VII ASSAY u/dL 30 FACTOR VIII ASSAY u/dL 7 Accredited Needs to be Accredited delivered Accredited directly to the coagulation laboratory Stored for up to 6 months in -70oC Freezer Accredited Accredited within 30 Accredited minutes of Accredited venesection Accredited G3.7a Revision 25 Page 16 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE FACTOR VIII INHIBITOR Bethesda 30 FACTOR IX ASSAY u/dL 30 FACTOR XIII SCREEN 30 HEPARIN (APTR) Ratio <1 HEPARIN-INDUCED (HIT) SCREEN THROMBOCYTOPENIA Pos/Neg 1 ACTIVATED PARTIAL THROMBOPLASTIN RATIO (APTR) ratio <1 INR - INTERNATIONAL NORMALISED RATIO ratio <1 FIBRINOGEN (DERIVED) g/L <1 LUPUS ANTICOAGULANT Pos/Neg 2 WARFARIN (INR) Ratio <1 Revision 25 G3.7a Accredited Needs to be delivered Stored for up to 6 directly to the months in -70oC coagulation Freezer laboratory within 30 minutes of venesection Accredited 2 x 2.7ml citrate 6mL Plain clotted 2 x 2.7 ml citrate 1 day 1 day 1 day 1 day 1 day 1 day 1 day Accredited Accredited Accredited Accredited Accredited Not Accredited Accredited Accredited Page 17 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE THROMBOPHILLIA SCREEN must be authorised by Haematology medic Includes; Clotting screen, APCR, Protein C &S, Antithrombin, Thrombin Time See individual tests 30 ANTITHROMBIN IU/dL 30 PROTEIN C IU/dL 30 1 x EDTA, Accredited 4 x citrate Stored for up to 6 Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection G3.7a Revision 25 Page 18 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE PROTEIN S IU/dL 30 THROMBIN TIME Ratio 30 THROMBOELASTOGRAPH (TEG) 1 VON WILLEBRAND SCREEN Includes; clotting screen, Factor CBA & RiCof if indicated VIII, VWF Ag, See individual tests 30 Revision 25 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection Stored for up to 6 Accredited 1 x citrate months in -70oC Freezer 2 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection 4 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection G3.7a Page 19 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE VON WILLEBRAND ANTIGEN IU/dL 30 ADAMTS13 (also known as von Willebrand Cleavage Protease) % (IU/dL) 1 COLLAGEN BINDING ASSAY (CBA) IU/dL 30 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection 1 x citrate Accredited Needs to be delivered directly to the coagulation laboratory within 30 minutes of venesection 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection G3.7a Revision 25 Page 20 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a RISTOCETIN CO-FACTOR ASSAY (RICOF) IU/dL PFA-200 seconds Platelet Aggregation Screen DEPARTMENT/TEST Units BLOOD TRANSFUSION Units ANTIBODY SCREEN (AUTOMATED) BLOOD GROUP (AUTOMATED) AUTOMATED GROUP & SCREEN Revision 25 30 1 1 TAT days(working) TAT days(working) 1 1 1 1 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection 2 x citrate Stored for up to 6 Accredited Needs to be months in -70oC delivered Freezer directly to the coagulation laboratory within 30 minutes of venesection Must be by prior arrangement with laboratory 4xcitrate samples must be delivered straight to lab Accredited Special Lab sample requirements storage time Accreditation Status Special requirements 6mL EDTA 10 days Accredited 6mL EDTA 10 days Accredited 6mL EDTA 10 days Accredited Page 21 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a FMH ml GROUP AND DIRECT ANTIGLOBULIN TEST (DAT) CROSS MATCH DIRECT ANTIGLOBULIN TEST (DAT) Foetal Maternal Haemorrhage Estimation (FMH) INVESTIGATION OF TRANSFUSION REACTIONS ANTI-A/ANTI-B TITRES Revision 25 3 or 6 ml EDTA TUBE Cord sample: 10 days 6mL EDTA 1 Neonatal sample: 0.5mL 6mL EDTA 10 days Based on clinical need. If atypical antibodies requires 1-2 working days 1 2 To be received in advance of blood requirement complying with BCSH sample validity guidelines 6mL EDTA 6mL EDTA 10 days 10 days See Transfusion Record for details 10 days Variable 2 To be sent upon discussion with laboratory ASAP after suspected reaction 6mL EDTA 10 days Accredited Accredited Accredited Accredited Accredited Accredited Page 22 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE ANTENATAL ANTIBODY TITRE OR QUANTITATION 10 6mL EDTA ANTIBODY INVESTIGATION & EXTENDED PHENOTYPING 1 6mL EDTA CELL FREE FOETAL DNA TESTING 16 6ml EDTA ALL TESTS LISTED BELOW MUST BE AUTHORISED BY A HAEMATOLOGY MEDIC HEPARIN-INDUCED THROMBOCYTOPENIA (HIT) ELISA (Confirmatory test) 1 6mL Plain clotted HLA ANTIBODY SCREEN HLA CLASS 1 TYPING FOR PLATELET REFACTIVENESS 13 10mL Plain clotted 2 x 6mL 13 EDTA 10mL Plain clotted PLATELET ANTIBODIES (AUTO OR ALLO) 3 x 6mL EDTA 10mL Plain clotted 10 If drug- related, a sample of drug is required Referred Accredited 10 days Accredited testing carried out if antibody screen is positive Referred Accredited 10 days Accredited Referred Referred Accredited Accredited Referred Accredited G3.7a Revision 25 Page 23 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE FOETAL/NEONATAL ALLO-IMMUNE THROMBOCYTOPENIA 10 NEONATAL ALLO-IMMUNE NEUTROPENIA 30 NEUTROPHIL/GRANULOCYTE ANTIBODIES 30 DONATH LANDSTEINER 10 Revision 25 Maternal: 6mLReferred EDTA 10mL Plain clotted Paternal: 6mL EDTA Neonatal: 1mL EDTA Maternal: 6mLReferred EDTA 10mL Plain clotted Paternal: 6mL EDTA Neonatal: 1mL EDTA 10mL Plain Referred clotted 6mL Plain clotted plus 6mL EDTA To be arranged with Lab for transport at 37’c. Must arrive within 30 minutes of venesection at 37’c Accredited Accredited Accredited Accredited G3.7a Page 24 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a HERIDATRY SPHEROCYTOSIS (EMA BINDING) 3ml EDTA Referred Accredited 7 To be sent mon-wed only with required blood film DEPARTMENT/TEST Units TAT Special days(working) requirements IMMUNOLOGY 6 ML SERUM SEPARATOR GEL (SST BUFF TOP) BLOOD SAMPLE FOR ALL TESTS UNLESS OTHERWISE STATED ACETYL CHOLINE RECEPTOR ANTIBODY nmol/L 20 Sent away if results are borderline ADRENAL ANTIBODIES Pos/Neg 15 ACLA (ANTI CARDIOLIPIN ABS) u/mL 10 B2GP1 reflex tested if pos ALLERGEN SPECIFIC IGE (RAST) kUA/L 5 ANA (see CONNECTIVE TISSUE ANA SCREEN) ANCA (ANTINEUTROPHIL ANTIBODY) CYTOPLASMIC Pos/Neg 5 ANTI COLLAGEN TYPE 2 (send away) Pos/Neg 20 ANTI CHOLINESTERASE (send away) Pos/Neg 20 AMYLOID –A (send away) Pos/Neg 20 ANTI C1Q (send away) Pos/Neg 20 ANTI-GLUTAMIC DECARBOXYLASE U/mL 20 ANTI-IGA (send away) Pos/Neg 20 ASPERGILLUS PRECIPITINS mg/L 10 AVIAN PRECIPITINS mg/L 10 Lab Storage time Accreditation Status Accredited Accredited Accredited Not Accredited Accredited Not Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Revision 25 Page 25 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE BENCE JONES PROTEIN present/absent 10 BETA 2 MICROGLOBULIN mg/L 5 BETA 2 GLYCOPROTEIN u/mL 15 C1 ESTERASE INHIBITOR (ANTIGENIC) g/L 10 C1 ESTERASE INHIBITOR (FUNCTIONAL) % 20 C3 NEPHRITIC FACTOR (sent away) Pos/Neg 20 CALPROTECTIN ug/g 15 CARDIAC MUSCLE ANTIBODY (send away) Pos/Neg 20 CARTILAGE AUTOANTIBODY (send away) Pos/Neg 20 CENTROMERE ANTIBODIES Pos/Neg 5 CCP ANTIBODY u/mL 5 COMPLEMENT C3/C4 g/L 5 SPECIALIST COMPLEMENT COMPONENTS (Send away) 20 COMPLEMENT PATHWAY FUNCTION CH50 % 20 ALTERNATIVE AP50 COMPLEMENT PATHWAY % 20 Revision 25 10 ML URINE (MSU) SAMPLE NEEDS FREEZING WITHIN 6 HOURS Only sent away for testing if C4 is normal range and C3 is below normal FAECAL SAMPLE SAMPLE NEEDS FREEZING WITHIN 6 HOURS SAMPLE NEEDS FREEZING WITHIN 6 HOURS SAMPLE NEEDS G3.7a Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Not Accredited Accredited Page 26 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE ALTERNATIVE FACTOR H COMPLEMENT PATHWAY mg/L 20 CONNECTIVE TISSUE ANA SCREEN Pos/Neg 5 COMPONENT RESOLVED DIAGNOSIS interpretive 25 CRYOGLOBULIN pos.neg 10 DNA ANTIBODIES IU/mL 5 ENA ANTIBODY SCREEN Pos/Neg 5 ENA ANTIBODY PROFILE Pos/Neg 5 ENA EXTENDED SCREEN Pos/Neg 15 EXTENDED LIVER AUTOANTIBODY BLOT Pos/Neg 10 ENDOMYSIAL ANTIBODIES Pos/Neg 15 FARMERS LUNG PRECIPITINS mg/L 10 FREE LIGHT CHAINS/LIGHT CHAIN RATIO mg/l 20 GANGLIOSIDE AUTO ANTIBODY Pos/Neg 20 GLOMERULAR BASEMENT MEMBRANE ANTIBODY U/mL 5 HEP2 SCREEN interpretive 10 HISTONE AUTOANTIBODY U/mL 20 HIB FUNCTIONAL ANTIBODYS mg/L 15 IMMUNOGLOBULINS + SERUM ELECTROPHORESIS g/L 5 IMMUNOGLOGULIN E (TOTAL) ku/L 5 IMMUNOGLOBULIN G SUBCLASSES g/L 20 IMMUNOGLOBULIN A –LOW CONCENTRATION g/L 20 Revision 25 FREEZING WITHIN 6 HOURS SPECIALIST REQUEST, IMM CONSULTANTS ONLY MUST BE KEPT AT 37C PRIOR TO SEP’N REFLEX TEST REFLEX TEST G3.7a Accredited Accredited Accredited Not Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Page 27 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE IMMUNOFIXATION INTRINSIC FACTOR ANTIBODIES KAPPA INDEX (CSFAND SERUM) LIVER AUTOANTIBODY SCREEN interpretive report 10 U/ml 10 mg/L and Index 15 Pos/Neg 5 MAST CELL TRYPTASE u/ml 10 MITOCHONDRIAL (M2) ANTIBODIES u/ml 10 MYELOPEROXIDASE (MPO) ANTIBODIES u/ml 5 MUSCLE SPECIFIC KINASE AUTOANTIBODY (send away) Pos/Neg 20 MYELIN ASSOCIATED GLYCOPROTEIN (send away) BTU 20 MYOSITIS Pos/Neg 20 NMDA Pos/Neg 20 NUCLEOSOME AUTOANTIBODY U/mL 20 OLIGOCLONAL BANDS interpretive result 10 OVARIAN AUTOANTIBODY Pos/Neg 20 PARATHYROID AUTOANTIBODY (send away) Pos/Neg 20 PEMPHIGOID/PEMPHIGUS (SKIN) ANTIBODIES Pos/Neg 10 PNEUMOCOCCAL (functional antibodies) mg/ml 15 PREVNAR SEROTYPES 20 PROTEINASE 3 (PR3) ANTIBODIES u/ml 5 Revision 25 REFLEX TEST Paired CSF and Serum samples required 2 samples req’d. 1st at event, 2nd 24hrs post event SEND AWAY REFLEX TEST Paired serum/CSF sample required SEND AWAY G3.7a Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Page 28 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE PURKINJE ) CELL AUTOANTIBODY (Hu,Yo Pos/Neg 15 PARANEOPLASTIC AUTOANTIBODIES Pos/Neg 25 RHEUMATOID FACTOR QUANTITATION iu/mL 5 SCANNED MONOCLONAL COMPONENT g/L 5 SCLERODERMA Pos/Neg 20 SKELETAL MUSCLE ANTIBODIES Pos/Neg 10 TETANUS ANTIBODIES iu/ml 10 THYROID PEROXIDASE ANTIBODIES u/ml 5 TISSUE TRANSGLUTAMINASE ANTIBODIES IgA u/ml 5 TISSUE TRANSGLUTAMINASE ANTIBODIES IgG u/ml 5 VGCC ANTIBODIES pmol/L 20 VGKC ANTIBODIES pmol/L 20 Total protein essential SEND AWAY SEND AWAY Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited Accredited G3.7a Revision 25 Page 29 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE G3.7a DEPARTMENT/TEST Units FLOW CYTOMETRY TAT Special Lab Storage time Accreditation Status days(working) requirements LEUKAEMIA/LYMPHOMA SCREEN interpretive report Lymphocyte Subsets (T, B and NK Subsets) Interpretive comment Neutrophil Oxidative Burst Interpretive comment PNH Screen Interpretive comment Neutrophil Phagocytosis Interpretive comment T cell receptor (TCR) repertoire (TCR V) EDTA 4.5 mL or Bone Marrow, <72 5 hrs old Monday – Friday 12:00 pm EDTA 4.5 ml, <72 10 hrs old Monday – Friday 12:00 pm Lithium Heparin 3ml <48 hrs old 5 Monday – Wednesday By appointment only EDTA 4.5 ml, <72 5 hrs old Monday – Friday 12:00 pm Lithium Heparin 3ml <48 hrs old 5 Monday – Wednesday By appointment only EDTA 4.5 ml, <72 4 hrs old Monday – Friday 12:00 pm Not Accredited Revision 25 Page 30 of 31 University Hospital Southampton NHS Foundation Trust LABORATORY MEDICINE Memory B cell subsets (CVID) Memory T cell Subsets Hairy Cell Leukaemia Screen T Cell Subsets (CD4 count) Therapeutic Antibody Evaluation Rituximab (Rituxan) (CD20) % B memory Subsets % T memory Subsets interpretive report % & Abs count B cells % & Abs Count EDTA 4.5 ml, <72 10 hrs old Monday – Friday 12:00 pm EDTA 4.5 ml, <72 10 hrs old Monday – Friday 12:00 pm EDTA 4.5 ml, <72 5 hrs old Monday – Friday 12:00 pm EDTA 4.5, <72 hrs old 5 Send FBC result. Monday – Friday 12:00 pm EDTA 4.5, <72 hrs 5 old Monday – Friday 12:00 pm • Test turnaround times defined by National Test directory at NHSE G3.7a Revision 25 Page 31 of 31
Url
/Media/UHS-website-2019/Docs/Services/Pathology/Lab-med/Laboratory-medicine-investigations.pdf

How to perform a breath test for small intestinal bacterial overgrowth (SIBO) at home - patient information

Description
This factsheet explains what small intestinal bacterial overgrowth (SIBO) is and how to perform a breath test for it at home.
Url
/Media/UHS-website-2019/Patientinformation/Digestionandurinaryhealth/How-to-perform-a-breath-test-for-SIBO-2892-PIL.pdf

Self-taken vaginal swab - patient information

Description
This factsheet explains how to collect a vaginal swab.
Url
/Media/UHS-website-2019/Patientinformation/Skin/Self-taken-vaginal-swab-patient-information.pdf

WGLS (combined) user handbook

Description
University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Wessex Genomics Laboratory Service Wessex Genomics Laboratory Service (WGLS) provides a comprehensive range of genomics services to the Trust, general practitioners and also to other external NHS and private sector organisations. Contents: - click the links or scroll down the page Contents Wessex Genomics Laboratory Service............................................................................................. 1 Key contacts.................................................................................................................................. 2 About our services ....................................................................................................................... 2 Service hours ................................................................................................................................ 2 Services offered............................................................................................................................ 2 Postal Address.............................................................................................................................. 3 Consent.......................................................................................................................................... 3 Service Charges ........................................................................................................................... 3 Information Governance.............................................................................................................. 4 User Feedback ............................................................................................................................. 4 Dealing with Complaints.............................................................................................................. 4 Availability of clinical advice........................................................................................................ 4 Completion of the request form.................................................................................................. 4 Specimen Collection .................................................................................................................... 5 Special advice on sample collection.......................................................................................... 5 Results Reporting......................................................................................................................... 6 Quality Assurance ........................................................................................................................ 6 Laboratory Departments..................................................................................................................... 8 Referral Forms ................................................................................................................................ 11 Other Documents:............................................................................................................................. 11 Page 1 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Key contacts Salisbury Laboratory Email Enquiries Telephone Enquiries Southampton Laboratory Email Enquiries Telephone Enquiries Other Key Contacts WGLS Operations Manager WGLS Quality Manager shc-tr.WRGLdutyscientist@nhs.net 023 81 207100 WGLS_cancergenomics@uhs.nhs.uk 023 81 206638 Charlotte.beard@uhs.nhs.uk Bethany.broadbent@uhs.nhs.uk About our services Wessex Genomics Laboratory Service (WGLS) is part of the Central and South Genomics Laboratory Hub. We provide an accredited genetics and genomics service for a core population of 3 million in the South Central (formerly Wessex) region of England, as well as specialist testing for other centres as part of the NHS Genomic Medicine Service (GMS). We accept samples referred by health care professionals from the UK or abroad, and offer high-quality testing, analysis, interpretation and reporting for a wide range of genetic conditions. Service hours Monday to Friday, 9am to 5pm (Southampton Site) Monday to Friday, 9am to 5:30pm (Salisbury Site) Closed Weekends and Bank Holidays Services offered From April 1st 2021, NHS genetic testing in England is specified by the National Genomic Test Directory and referral criteria should comply with the associated eligibility criteria. Referrals outside the scope of the Test Directory and its associated criteria will not be funded by NHS England and will be subject to a charge. Page 2 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Postal Address Salisbury: Salisbury District Hospital (SDH North) Salisbury Wiltshire SP2 8BJ Southampton: Duthie Link Building, Mail point 225 University Hospital Southampton NHS Foundation Trust Tremona Road Southampton Hampshire SO16 6YD Consent Please see the following document available on the UHS website: Consent to Examination or Treatment: Policy Patients attending venesection services will be asked to give verbal consent prior to blood specimens being collected. All consent is inferred by the laboratory when a sample is submitted having been willing collected from the patient, for example venipuncture or biopsy. In addition to the above, the WGLS (Salisbury) referral forms include the statement “in submitting this sample the clinician confirms that consent has been obtained for testing and storage. Anonymised stored samples may be used for quality control procedures including validation of new genetic tests”. As a result, for any samples received on a WGLS referral form, consent for the testing requested is assumed and that the responsibility of obtaining consent lies with the referring clinician. (This, however, does not preclude the laboratory from contacting the referring clinician, even if consent is evident, if there is a question regarding the appropriateness of the requested test). At WGLS we understand the importance of obtaining consent for genomic testing and the consequences of proceeding with genetic analysis in its absence. Any issues relating to consent are picked up at the sample investigation review stage. Service Charges Each request accepted by the laboratory for examination(s) shall be considered an agreement with the sender. Referrals between NHS England according to the National Test Directory will not be subject to direct payment. Services provided outside of the National Test Directory and services provided to Page 3 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 requestors outside of NHS England will be subject to charge and invoiced on completion of work. Details of test pricing is available on request and service level agreements should be requested. Information Governance All staff working for the Pathology have a legal duty to keep information about patients and staff members confidential and to protect the privacy of individuals. All staff adhere to the Trust’s Data Protection and Confidentiality Policy and are mandatorily required to perform annual Information Governance training. User Feedback We welcome user feedback and appraisal of our service by emailing shctr.WRGLdutyscientist@nhs.net or WGLS_cancergenomics@uhs.nhs.uk and the service has a policy for seeking user feedback by survey. Dealing with Complaints WGLS adheres to the Trust Policy for handling concerns and complaints. All complaints, either raised via Patient Support Services or directly to a member of staff from within the department will be thoroughly investigated and actioned to resolve any identified issues. Availability of clinical advice Clinical Scientists are available during routine working hours to provide help with the interpretation of results and other clinical advice. Completion of the request form The appropriate request form should be downloaded from this site and properly completed. A request form must accompany all specimens sent to the laboratory and should clearly state the following information: • Surname and forename • Hospital /NHS Number • Date of birth • Sex • Ward/Clinic and Consultant code • Type of specimen • Date and time of collection • Investigations required including National Genomic Test Directory Code Page 4 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 • Relevant clinical information with evidence for clinical utility where appropriate • Identification of priority status with reasons for urgency based on clinical need Specimen Collection Samples should be collected into appropriate tubes and sent to the laboratory. Please allow tubes to fill to capacity. Pathology Receptions at Salisbury and Southampton Hospitals are open and able to receive samples 24 hours a day, 7 days a week. Samples should be clearly labelled with at least three points of patient identification. A request form that provides patient information as listed above should accompany samples. Special advice on sample collection Specimen rejection Specimens will be rejected if they are unsuitable for the investigations requested or if the identity of the patient is in doubt. High risk specimens and safety All specimens must be collected into leak resistant containers. The container must be appropriate for the purpose, properly closed and not contaminated on the outside. All specimens are regarded as high risk, but if they are taken from a patient who is known to be infected with a blood-borne agent such as Hepatitis B virus and HIV, another serious infectious disease such as tuberculosis or typhoid, or from those at risk of being infected by one of these agents, then extra care should be taken to highlight this. These specimens should be labelled as HIGH RISK on the request form. Specimen transport All sample containers from a single request are to be sealed into a clear plastic specimen bag by the person taking the sample. Specimen request forms/support documents must not be placed in the same compartment as the sample. UHS specimen transport arrangements: Samples are collected from wards on a frequent basis by the portering service. UHS GP Practice specimen transport and collection arrangements: Samples are collected from surgeries and clinics on a daily basis. For details of frequency and times please contact: Transport Department 140 Mauretania Road Nursling Industrial Estate Page 5 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Southampton SO16 6YS Tel: 023 80748027 Postal/Courier Referrals from Other Laboratories: All referrals if sent by road must be sent in accordance with UN 3373 Biological Substance Category B Packing instruction P650. Please send all referral samples using the postal/courier service of your choice. Results Reporting Validated results are reported electronically to UHS results servers eQuest and ICE. Hard copy reports for valid locations are printed and dispatched every working day either by post or email. Quality Assurance We are a ISO15189 accredited medical laboratory service committed to providing users with a service of the highest quality to meet clinical need. We operate a comprehensive quality management system and aim to produce: • Accurate results • Using appropriate testing strategies • In an appropriate timeframe • With appropriate comment and interpretation to assist clinicians in providing the best management of their patients. Our tests are all fully validated and/or verified internally (as appropriate) to include sensitivity, specificity, measurement uncertainty (where applicable), internal quality control and test limitations/interferences. Test limitations and sensitivity are stated on our reports, where appropriate. Information relating to any of the above validation parameters is available on request. As part of our ongoing commitment to quality standards, we participate in all relevant external quality assessment schemes provided by the following EQA bodies: GenQA (formerly UK NEQAS for Molecular Genetics and CEQAS); Leucocyte Immunophenotyping; Blood Coagulation; Histocompatibility and Immunogenetics; European Molecular genetics Quality Network (EMQN), European Research Initiative CLL (ERIC) and EuroClonality for all of which we typically achieve excellent results. Please contact us for further details if required. Page 6 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 We aim to follow all national best practice guidelines and endeavour to meet NHSE recommended turnaround times. Please consult the National Turn Around Targets to find the reporting times you can expect for your referrals. Quality management, accreditation and external quality assessment Wessex Genomics Laboratory Service (Salisbury) UKAS reference number: 9055 The majority of the tests offered by the laboratory are accredited to ISO15189:2022, Tests currently provided outside the UKAS schedule of accreditation: • SNP array (pending Extension to Scope) • Whole Genome Sequencing (pending Extension to Scope) • FLT3-TKD hot spot by fragment analysis (pending Extension to Scope) • Pan-Haematological NGS panel (pending Extension to Scope) • NGS assays on DNA extracted from formalin-fixed paraffin-embedded (FFPE) material (pending Extension to Scope) • Monitoring of FIP1L1::PDGFRA fusion and other rarer gene fusions associated with eosinophilic MPNs by nested RT-PCR or multiplex genomic DNA analysis (pending Extension to Scope) Wessex Genomics Laboratory Service (Southampton) UKAS reference number: 9194 The majority of the tests offered by the laboratory are accredited to ISO15189:2022, Tests currently provided outside the UKAS schedule of accreditation: • Actionable Solid Organ Panel (pending extension to scope) The full UKAS ISO15189 schedules of accreditation are detailed on the UKAS website https://www.ukas.com/find-an-organisation/ Page 7 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Laboratory Departments Oncology Genetics (Salisbury and Southampton Sites) We offer a comprehensive service for the diagnosis and monitoring of a wide range of haematological malignancies, solid tumours and an expanding number of hereditary and somatic mutation cancers using conventional cytogenetics, FISH and molecular techniques as appropriate. For details, please use the appropriate link: • Sample requirements • Sample prioritisation and reporting times • Full list of tests according to disease subtype Contact: Laura Chiecchio (Salisbury) l.chiecchio@nhs.net Nicola Meakin (Southampton) nicola.meakin@uhs.nhs.uk Pharmacogenomics A range of pharmacogenomic tests are offered by the service including DPYD genotyping. Contact: Nicola Meakin (Southampton) nicola.meakin@uhs.nhs.uk Page 8 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Rare and Inherited Disease Genetics (Salisbury) For a full list of tests conducted by WGLS at Salisbury, see our Rare Disease service list. Samples referred for tests not undertaken at our laboratory may be sent to other accredited centres either within or outside the Central and South Genomics Laboratory Hub. We are also happy to accept private and overseas referrals. Please contact Duty Scientist for details and prices. Pregnancy Loss and Solid Tissue Service (Salisbury) Testing is available for referrals that meet the laboratory’s acceptance criteria, with a tiered testing approach dependent upon the referral details and patient’s obstetric history. Referrals are accepted for: • Pregnancy loss or termination with significant fetal malformations (irrespective of gestation). • Pregnancy loss > 24 weeks. Also, in line with the published guidelines of the Royal College of Obstetricians and Gynaecologists (RCOG): • Miscarriages (<24 weeks) for 3rd and subsequent miscarriages. In compliance with RCOG guidelines the laboratory does not routinely accept referrals for parental karyotyping for couples experiencing recurrent miscarriages. Full details of our referral acceptance policy and the available tests are summarised in our solid tissue service guide. Molecular genetics (Salisbury) A wide range of rare disease molecular genetic tests is conducted within our GLH, including nextgeneration sequencing panels, single gene screens, targeted testing for known or common pathogenic variants, analysis of triplet repeat expansions, methylation studies for imprinting disorders and Xinactivation studies. Please note that from 1st July 2021, testing for certain clinical indications' will be carried out by whole-genome sequencing (WGS). Referrals for carrier testing or pre-symptomatic testing for late-onset genetic disorders will only be accepted from a Clinical Geneticist. Cytogenetics (Salisbury) Constitutive cytogenetic testing investigates the whole genome for loss or gain of chromosomal material and structural rearrangements involving chromosomes in neonatal, paediatric, adult and perinatal cases. A range of tests is conducted at WGLS: Page 9 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 • Microarray Genome-wide chromosomal microarray (CMA) testing (refer to our User guide) detects copy number variants (CNVs). It is a high-resolution test used for patients with, dysmorphism or certain congenital abnormalities, and for fetal losses with specified clinical indications. • Karyotyping Conventional G-banded karyotype analysis is provided for certain referral indications e.g. where a balanced chromosome rearrangement, sex chromosome imbalance, mosaicism, or common trisomy is suspected. It is also commonly used as a reflex test for characterisation of chromosome abnormalities detected by other techniques and for follow-up studies to establish recurrence risks. • Fluorescent in situ hybridization (FISH) FISH testing can detect a gain, loss or rearrangement of a specific region of interest. It is a targeted reflex test for the characterisation of chromosome abnormalities detected by other techniques and for follow-up studies to establish inheritance. • Quantitative fluorescent PCR (QF-PCR) This test is primarily used for the rapid diagnosis of common chromosome aneuploidy syndromes in fetal losses, neonates and paediatric cases, or to determine the genetic sex of a patient. Rare Disease Contacts: simon.thomas1@nhs.net (Molecular Genetics) caroline.price4@nhs.net (Cytogenetics) Page 10 University Hospital Southampton NHS Foundation Trust DOC987 Revision 1/ WRGL 33687 V1.0 Referral Forms Rare and Inherited Disease referral form Rare and Inherited Disease referral form RNA analysis referral form BRCA Mainstreaming referral form Oncology Genetics (Salisbury) Oncology referral form Oncology Genetics (Southampton) MPF9 Solid tumour request form MPF12 HaemOnc request form Pregnancy and Solid Tissues Solid Tissues referral form SPIRE Referrals SPIRE referral form Other Documents: WRGL Website Oncology Guide on Sample Requirements WRGL Website Oncology Guide on Sample Prioritisation WRGL Website Oncology Tests Performed According to Disease Entity WRGL Website Solid Tissue Service Guide Clinicians Guide to the Genomic Medicine Service Requesting Genomic tests using the National Genomics Test Directory and PanelApp for Rare Disease Rare Disease service list WRGL Website Microarray User Guide Page 11
Url
/Media/UHS-website-2019/Docs/Services/Pathology/WGLS-combined-user-handbook.pdf
1 to 10 of 259
Previous 1 2 3 4 5 … Next