Diagnostic tests and investigations (Q-T)

  • This is an alphabetical listing of all the diagnostic tests offered by the laboratory.
  • This consists of a mixture of "in-house" tests performed on-site and specialist tests that are sent to a reference laboratory.
  • Turnaround times will vary depending on the test and where it is performed.
  • For best results, please ensure the correct type of specimen is sent in the correct container.
  • Please note that availability for some tests is restricted and requires prior discussion/ authorisation by a microbiologist/ virologist.
  • For electronic requesting via e-QUEST: we have endeavoured to provide e-QUEST testing codes [in brackets] where possible. 
  • If unsure as to how to interpret a result, contact us. Please be ready with a full clinical history, up to date clinical data and recent antimicrobial treatment details before you call.
A-Z of Diagnostic Tests and Investigations

Test

Specimen

Container

Required volume

Laboratory

Turnaround time

Additional information

[Q fever reference test]

See under Coxiella burneti

Rabies investigations

Discuss with a virologist

Rash (viral serology screen)

[e-QUEST: Rash non-vesicular]

ASO titre

Parvovirus IgM, IgG

Rubella IgM, IgG

[e-QUEST: Rash vesicular]

Varicella IgG

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

2-3 days

Please state type and distribution of rash- this dictates which tests are performed.

Please state if patient is pregnant.

Respiratory syncitial virus (RSV) IF/ PCR

See under Respiratory viruses PCR/ NPA

Respiratory virus PCR

[e-QUEST: Influenza A&B/ Parainfluenza/ RSV/SARS-CoV-2 Adenovirus/Metapneumovirus/Rhinovirus]

Respiratory secretions

NPA, BAL, Sputum, Swab in viral transport medium.

Sterile Universal

2 ml (min vol 200µl)

SGH

2-3 days

Tests run more frequently during peak influenza season

Rickettsial serology- Typhus or Spotted Fever

[e-QUEST: Rickettsial serology]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

5 days

Discuss with microbiology.

Provide a full travel history.

Ross River virus serology

See under Arboviral serology

Rotavirus

See under Faeces Virology investigations

Rubella serology (acute)

[e-QUEST: Rubella IgM]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days

Please state if patient is pregnant.

Rubella serology (immunity)

[e-QUEST: Rubella IgG screen]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days

 

Salmonella serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

17 days

Requires prior discussion with a microbiologist

SARS-CoV-2 PCR (Routine Testing)

Swab in viral transport medium

Swab in viral transport medium

1 ml

SGH

1 day

 

SARS-CoV-2 PCR (Rapid Testing)

Swab in viral transport medium

Swab in viral transport medium

1 ml

SGH

4 hrs

Bleep 2066 for triage.

SARS-CoV-2 (Fast-track)

Swab in viral transport medium

Swab in viral transport medium

1 ml

SGH

5-6 hrs

Please bring samples to Microbiology department, Level B, and leave in drop-box.

SARS-CoV-2 Antibody IgG

Blood

(serum)

Clotted Blood (red top tube) 5-10 ml SGH 1-2 days  

Schistosomiasis microscopy

Faeces

Sterile Universal

1-2 gm

With the spatula provided transfer a plum-sized portion of faeces

SGH

7 days

 

 

 

 

 

2 days

3 specimens on 3 consecutive days – give clinical details

Urine (3 consecutive terminal urine samples collected at midday)

Sterile Universal

10 ml

Schistosomal serology

[e-QUEST: Schistosoma reference test]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

Send at least 6 weeks post-exposure.

Sellotape slide

See under Threadworms

Seminal fluid for MC&S

Seminal fluid

Sterile Universal

  N/A

SGH

1-2 days

 

Shigella serology

[e-QUEST: Shigella Serology]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

14 days

Requires prior discussion with a microbiologist

Shingles

See under Varicella

Sleeping sickness

Send blood films for diagnosis of acute infection, otherwise see Trypanosomiasis serology

Sputum/ ETA/ BAL/ NBL

[eQUEST: Sputum/ETA/BAL etc]

Respiratory

Sterile Universal

5-10 ml

SGH

2-3 days

Please refer to Mycobacteria for TB investigation.

Sputum (Cystic)

Cystic Sputum/cough swab)

Respiratory

Sterile Universal

5-10 ml

SGH 6-8 days

Please refer to Mycobacteria for TB investigation.

Staphylococcal serology

[e-QUEST: Staphylococcal reference test]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

14 days

Requires prior discussion with a microbiologist

Staphylococcal (MSSA/ MRSA) PCR

Tissue/ Pus/ Swabs/ Fluids

Requires prior discussion with a microbiologist

Stem cell transplant screen

[e-QUEST: Stem cell transplant screen]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

7 days

Consists of:

CMV IgG

EBV (EBNA IgG)

HIV Ab/Ag

HTLV 1+2 Ab

Hepatitis B Core Ab

Hepatitis BsAg

Hepatitis C IgG

Syphilis IgG

Toxoplasma IgM, IgG

Varicella IgG

Streptococcal serology (ASOT, Anti DNAse B)

See under ASO titre/ Anti Streptolysin O and Anti DNAse B titre

Strongyloides microscopy

See Faecal Parasitology/ OCP (may need up to 6 samples as is insensitive)

Strongyloides serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

 

Supra-pubic aspiration (SPA)

See under Urine MC&S

Swabs for MC&S (skin/ wound etc)

Swab in Amies transport medium with charcoal.

N/A

N/A

SGH

4 days

Do not request MRSA swabs unless screening.

Syphilis serology

[e-QUEST: Syphilis antibody (blood)]

or

[e-QUEST: Syphilis antibody (CSF)]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-3 days

 

CSF (VDRL, TPPA)

Sterile Universal

1-2 ml

Reference

Tapeworms / Taenia spp

See Faecal Parasitology/ OCP

Taenia (cysticercal) serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

For diagnosis of cysticercosis.

TB investigations

See under Mycobacteria

Teicoplanin levels

See under Antibiotic Assays

Tetanus toxin serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

7-14 days

Collect before antitoxin given. Discuss with microbiology if suspected.

Tissue/ Biopsy for MC&S

See under Fluids

Thread worms

Place sellotape over perianal region

Transfer to clean microscope slide

N/A

SGH

1-2 days

Best taken early morning

Throat swabs

Swab in Amies transport medium with charcoal.

N/A

N/A

SGH

4 days

 

Tobramycin levels

See under Antibiotic Assays

[e-QUEST: TORCH screen (infant)]

For serological investigation of suspected congenital infection.

Toxoplasma IgM, CMV IgM, Rubella IgM, Syphilis

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days 

If not requesting via e-QUEST please state "suspected congenital infection" and give clinical details.

[e-QUEST: TORCH screen (mother)]

For serological investigation of suspected intrauterine infection.

Toxoplasma IgG & IgM, CMV IgG & IgM, Rubella IgG & IgM +/- Parvovirus IgG & IgM, Syphilis.

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

 1-2 days

If not requesting via e-QUEST please state "suspected intrauterine infection" and give clinical details including gestational age and any relevant exposures.

Toxocara serology

[e-QUEST: Toxocara reference test]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

 

Toxoplasma serology (past exposure)

[e-QUEST: Toxoplasma IgG]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days

Please state if patient is pregnant.

Toxoplasma serology (acute/ current/ recent infection)

[e-QUEST: Toxoplasma IgM]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days

Please state date of onset/ exposure.

Please state if patient is pregnant.

Toxoplasma PCR

Discuss with Virologist/ Microbiologist

Trichinella serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

 

Trypanosomiasis serology (Chagas' disease/ Sleeping sickness)

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

28 days

Send blood films for diagnosis of acute infection.

Tularaemia (Fransicella tularensis) serology

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

5 days

Requires prior discussion with a microbiologist

Typhus serology

See under Rickettsial serology