Diagnostic tests and investigations (U-Z)

  • This is an alphabetical listing of all the diagnostic tests offered by the laboratory.
  • This consists of a mixture of "in-house" tests performed on-site and specialist tests that are sent to a reference laboratory.
  • Turnaround times will vary depending on the test and where it is performed.
  • For best results, please ensure the correct type of specimen is sent in the correct container.
  • Please note that availability for some tests is restricted and requires prior discussion/ authorisation by a microbiologist/virologist.
  • For electronic requesting via e-QUEST: we have endeavoured to provide e-QUEST testing codes [in brackets] where possible.
  • If unsure as to how to interpret a result, contact us. Please be ready with a full clinical history, up to date clinical data and recent antimicrobial treatment details before you call.
A-Z of Diagnostic Tests and Investigations

Test

Specimen

Container

Required volume

Laboratory

Turnaround time

Additional information

Urine antigens (Pneumococcus/ Legionella)

Urine

Sterile Universal

10 –20ml

SGH

1-2 days

Needs prior authorization by a microbiologist.

Urine MC&S (MSU, CSU, Clean-catch, Bag Urine, Supra-pubic aspiration)

[e-QUEST: Urine MC&S]

Specimens will be rejected if there are inadequate clinical details.

Urine

Sterile Universal

10 –20ml

SGH

1-2 days

Specimens are rejected if >24 hrs as sub optimal for the isolation of organisms. They may be refridgerated overnight but not on Fridays, unless there is a Saturday collection.

Vaginal swabs MC&S

(HVS/ LVS)

Swab in Amies transport medium with charcoal.

N/A

N/A

SGH

2-3 days

 

Vancomycin levels

See under Antibiotic Assays

Varicella zoster (VZV) IgG

[e-QUEST: Varicella zoster IgG]

Not for diagnosis of acute infection- send vesicle fluid for isolation/ PCR.

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

1-2 days

Please state if the patient is pregnant and exposed to VZV as will need urgent testing

Varicella zoster (VZV) PCR (chickenpox/ shingles)

[e-QUEST: Varicella zoster PCR]

CSF

Sterile Universal

2 ml

SGH

1-2 days

 

Blood

EDTA blood

2 ml

Lesion/ Vesicle Swab

Swab in Viral Transport Medium

N/A

Vesicle fluid

Capillary tube

Min 200µl

Vascular access device tips

[e-QUEST: CVP/ Hickman/ cannula/ peripheral lines/ vascath etc]

Device

Sterile Universal

N/A

SGH

2-3 days

Will NOT be processed routinely.

Only where line sepsis is suspected.

VDRL slide test

[e-QUEST:Syphilis antibody (blood)]

OR

[e-QUEST:Syphilis antibody (CSF)]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

SGH

4 days

 

CSF

Sterile Universal

Vibrio cholerae & other species.

Faeces

Universal 30ml faecal pot

1-2gm

SGH

2-3 days

Please give travel history

Viral eye swabs

[e-QUEST:Viral eye swab]

HSV and Adenovirus PCR

Eye swab in VTM

Swab in Viral Transport Medium

  N/A

SGH

1-2 days  

Viral Haemorrhagic Fevers (VHF)

Discuss with a Virologist/ Microbiologist urgently

Viral meningo-encephalitis studies

[e-QUEST: Viral Menigitis/enceph.]

Includes:

HSV, Enterovirus and Meningococcal PCR

CSF

Sterile Universal

2 ml

SGH

1-2 days

Please give date of onset and any travel history.

Viral swabs (genital/ lesions/ vesicles)

Lesion swab

Swab in Viral Transport Medium

 

SGH

  1-2 days

Give full clinical details.

Vitreal biopsies/ tap

Vitreous fluid

   

SGH

 2 days

Please state clearly if fungal infection suspected.

VRE screen Rectal swab Sterile swab in Amies transport medium N/A SGH 2 days for negative, 3 days for presumptive positive Specimens are rejected if >48 hrs as sub optimal for the isolation or organism (patients from intestinal failure unit)

Weil's disease

See under Leptospirosis

West Nile virus

Please discuss with virology

Whipples PCR

Please discuss with microbiology

Whipworm (Trichuris trichiura)

See under Faecal Parasitology/ OCP

Wound swab for MC&S

(Do not request for MRSA unless sending screening swabs)

[e-QUEST: Wound/ear/nose/throat]

Swab in Amies transport medium with charcoal.

N/A

N/A

SGH

2-3 days

Specimens are rejected if >48 hrs as sub optimal for the isolation or organisms. May be stored overnight at room temperature if necessary.

Yellow fever virus serology

See under Arboviral serology. Discuss with a virologist.

Yersinia pestis isolation

(plague)

Discuss with a microbiologist urgently

Yersinia serology

[e-QUEST: Yersinia reference test]

Blood (serum)

Clotted Blood (red top tube)

5-10 ml

Reference

14 days

 

Ziehl-Nielsen (ZN) stain

See under Mycobacteria.